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Aquastat X11 —
The new standard in bacteria and mold protection

The control of bacteria and viruses has long been a primary concern in the protection of human health. More studies are being released everyday that show how germs (bacteria, viruses, mold and fungi) are able to stay alive and active on various surfaces. The innocent passerby then comes in contact with the contaminated surface and has now become a host for the bacteria or virus to grow and be transmitted to yet another person. As the process of transmission of illness is becoming understood more every day, there is a growing awareness of the need to control biological growth in and on surfaces. Some specific areas where surfaced-based antimicrobial protection is fey are: household and specialty products, medical devises, building materials, countertops and kitchen components, children’s toys, plastics and HVAC systems.

Despite the many products claiming the ability to protect surfaces from bacteria growth, independent studies are beginning to show that many of these products have no or little efficacy to control bacteria. Additionally, many products are limited in functionality in that they are designed to control bacteria growth alone, with no ability to control mold and fungi.

Aquastat X11To meet these challenges, we have transformed four years of concentrated research into a product with superior antimicrobial activity in an easy-to-apply form. Our patented antimicrobial additive is manufactured as a fine, inorganic, free-flowing powder that has been proven to control a broad spectrum of bacteria, mold and fungi. We also has the ability to custom-design the Aquastat X11 material for formulation into specific devices or end uses for a wide range of applications.

The engineered functionality behind the Aquastat material enables it to release trace amounts of our proprietary mineral-based antimicrobial when moisture is present (either though contact with a human hand, water, or humidity in the air). By functioning in this fashion, the media acts as a time released capsule maintaining a constant level of the antimicrobial protection on all surfaces to prevent bacteria and mold growth over extended periods. Additionally, the inorganic nature of the Aquastat X11 material allows safe handling, storage, and application into any current fabrication process.

The possibilities...

  • Air filtration systems for home, office, and confined areas
  • Humidification/dehumidification systems
  • Paper-based building materials
  • Paint and Surface coatings
  • Plastics and molded products
  • Fabrics
  • Medical devises
  • Counter tops and kitchen items
  • Children’s toys
  • Resin-based outdoor furniture

Benefits

  • Registered for use in air and drinking water filters
  • Controls a Broad Spectrum of bacteria, molds and viruses
  • Time Release action allows continuous protection in high humidity or even water environments.
  • US EPA Registered Product
  • Registered for use in drinking water filters.
  • Inorganic-based and stable – easily applied into various coating or admixing applications.

Bacteriostatic Evaluation of Paper Impregnated with Aquastat® X11 Over a Four-Week Period

by Sharifeh Mehrabi PhD. of Clark Atlanta University

Overview of experiment

Paper sheet containing Aquastat® X11 antimicrobial (lot#5822) manufactured by K2 Concepts, Inc was tested against standard (untreated) paper sheet for its ability to prohibit bacteria and fungi growth in paper over a 4- week period.

Samples (2 x 3 inches) were cut from the sheet of Aquastat® X11 impregnated paper as well as a control paper (untreated), weighed and placed standing on end in 200 mL beakers. 10 mL of a bacteria/fungi suspension, prepared from E coli, Flavobacterium (Chryseobacterium-indologenes) and a fungi (approximately 1000 CFU/mL) was added to beakers. The beakers were sealed with parafilm for 24 hours. The following day the parafilm was replaced with a petri dish to allow ventilation. Additional stock of bacteria/fungi suspension (1000CFU/mL) was added twice weekly to the beakers to replenish the stock (see figure 1).

Sampling procedure

One strip of approx. 1 cm width of each paper was cut and then divided into 4 pieces, top to bottom. The top and bottom of each paper sample was placed in a sterile tube with 2 mL of sterile CaCl2 solution and then sonicated for 10 minutes. A sample of 0.5 mL of the resulting solution (suspension) was added to a R2A agar plate and incubated for three days. Samples were taken and analyzed weekly.

Results

Week N°
Paper section
1 = Top
4 = Bottom
Sample #1
No Antimicrobial
Aquastat® X11
Sample
1
1
+++
F
0
4
+++
B,F
0
2
1
0
0-1
4
++++
B, F
0
3
1
+++++
B, F
0
4
+++++
B, F
0
4
1
++++
B, F
± B
4
++ B, F
± B, ± F
LEGEND B: Bacteria
F: Fungi
(++++) Heavy growth (TNTC)
(++) moderate growth
(+) slight growth
(±) 1-2 colonies

Special Note

After week 3 of the testing traces of bacteria colonies began to appear on all the R2A agar plates after 7 days of incubation. This bacteria species was isolated and identified by staining and found to be a gram positive spore forming Bacillus sp. Spore forming bacteria are well known for their ability to hibernate or become dormant while in a harsh or inhospitable environment. However, when these spores are transferred into a nutrient rich environment such as that found on the R2A agar plate, the spores become active and colonize. Due to the extended 7-day incubation period these Bacillus spores as found on the paper were thus not active while on the paper, but when relocated to the R2A agar became active. In spite of the presence of these spores, the Aquastat® X11 treated paper sample continued to control bacterial and fungal growth through the entire test period. Photo of Bacillus species is shown in Figure 1.

Conclusion

Results from the R2A agar plates indicate that the Aquastat® X11 impregnated paper controlled bacterial and fungal growth even after being contaminated by guest spore forming bacteria (Bacillus) while the untreated paper supported significant bacteria and fungal growth (see Figure 2). Additionally, distinct visible differences in the treated vs. untreated paper samples were observed. Pictures of the treated vs. untreated sample are shown in Figure 4.

Disclaimer | All results are based on independant study conducted by their noted authors. K2 Concepts, Inc maintains no involvment in these findings.


Figure 1


Figure 2
Bacteria/Fungi found on control sample vs. sample containing Aquastat® X11


Figure 3
Treated (left), un-treated (right)


Figure 4
Photo of Bacillus sp.

top left top right

Additional Aquastat X11 Resources

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Thank you for your interest in our ever expanding line of products.
K2 Concepts, Inc

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